２０１６年

Fortunato AE, Jaubert M, Enomoto G, Bouly JP, Raniello R, Thaler M, Malviya S, Bernardes JS, Rappaport F, Gentili B, Huysman MJ, Carbone A, Bowler C, d'Alcalà MR, Ikeuchi M, Falciatore A. (2016) Diatom phytochromes reveal the existence of far-red-light-based sensing in the ocean. Plant Cell, in press. [PubMed] フランス、Falciatore先生、Bowler先生らとの共同研究による珪藻フィトクロムの機能を解析した論文。

Nagao R, Tomo T, Narikawa R, Enami I, Ikeuchi M (2016) Conversion of photosystem II dimer to monomers during photoinhibition is tightly coupled with decrease in oxygen-evolving activity in the diatom Chaetoceros gracilis. Photosynthesis Research, in press. [PubMed] 長尾さん（現 名古屋大）の博士論文の一部をまとめた論文。

２０１５年

Song C, Velazquez Escobar F, Xu XL, Narikawa R, Ikeuchi M, Siebert F, Gärtner W, Matysik J, Hildebrandt P. (2015) A red/green cyanobacteriochrome sustains its color despite a change in the bilin chromophore's protonation state. Biochemistry. 54: 5839-48. [PubMed] 成川先生（静岡大）とHildebrandt先生（ベルリン工科大）の共同研究によるAnPixJ発色機構の解析論文。

Song C, Narikawa R, Ikeuchi M, Gärtner W, Matysik J. (2015) Color tuning in red/green cyanobacteriochrome AnPixJ: photoisomerization at C15 causes an excited-state destabilization. J Phys Chem B. 119: 9688-95. [PubMed] 成川先生（静岡大）とMatysik先生（ライデン大）の共同研究によるAnPixJ発色機構の解析論文。

Enomoto, G., Ni-Ni-Win, Narikawa, R. and Ikeuchi, M. (2015) Three cyanobacteriochromes work together to form a light color-sensitive input system for c-di-GMP signaling of cell aggregation. Proc. Natl. Acad. Sci. U S A. 112: 8082-8087. [PubMed]［プレスリリース］好熱性シアノバクテリアにおける３つの光受容体シアノバクテリオクロムの役割を生化学と変異体解析で明らかにした榎本君（D3）の労作。

Narikawa, R., Fushimi, K., Ni-Ni-Win, M. Ikeuchi (2015) Red-shifted red/green-type cyanobacteriochrome AM1_1870g3 from the chlorophyll d-bearing cyanobacterium Acaryochloris marina. Biochem. Biophys. Res. Comm. 461(2): 390-5. [PubMed] 成川先生（静岡大）のシアノバクテリオクロムの解析論文。

Narikawa, R., Nakajima, T., Aono, Y., Fushimi, K., Enomoto, G., Ni-Ni-Win, Itho, S., Sato, M., Ikeuchi, M. (2015) A biliverdin-binding cyanobacteriochrome from the chlorophyll d–bearing cyanobacterium Acaryochloris marina. Scientific Reports, 5. [PubMed] ［プレスリリース］成川先生（静岡大）の遠赤色光吸収光受容体（ビリベルジン結合シアノバクテリオクロム）を初めて報告しました。

Hirose, Y., Katayama, M., Ohtsubo, Y., Misawa, N., Iioka, E., Suda, W., Ohsima, K., Hanaoka, M., Tanaka, K., Eki, T., Ikeuchi, M., Kikuchi, Y., Ishida, M., Hattori, M. (2015) Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3709 that harbors phycoerythrin-rich phycobilisome. Genome Announcements, 3 (2): e00385-15. [PubMed] 広瀬先生（豊橋技科大）のゲノム解析論文。

Hirose, Y., Katayama, M., Ohtsubo, Y., Misawa, N., Iioka, E., Suda, W., Ohsima, K., Hanaoka, M., Tanaka, K., Eki, T., Ikeuchi, M., Kikuchi, Y., Ishida, M., Hattori, M. (2015) Complete genome sequence of cyanobacterium Geminocystis sp. NIES-3708, which performs type II complementary chromatic acclimation. Genome Announcements, 3 (3): e00357-15. [PubMed] 広瀬先生（豊橋技科大）のゲノム解析論文。

Maeda, K., Narikawa, R. and Ikeuchi, M. (2015) Measurement of nucleotide triphosphate sugar transferase activity via generation of pyrophosphate. Bio-Protocol, 5: e1450. 前田君（D1）の酵素活性測定法の論文。

Kuroi K, Okajima K, Ikeuchi M, Tokutomi S, Kamiyama T, Terazima M. (2015) Pressure-sensitive reaction yield of the TePixD blue-light sensor protein. J Phys Chem B. 119(7): 2897-907. [PubMed] 寺嶋先生（京大）のTePixD解析の共同研究論文。

２０１４年

Kuroi, K., Okajima, K., Ikeuchi, M., Tokutomi, S. and Terazima, M. (2014) Transient conformational fluctuation of TePixD during a reaction. Proc. Natl. Acad. Sci. U S A. 111: 14764-14769. [PubMed] 京大・寺嶋先生の光受容体の構造変化の解析の論文。

Midorikawa T, Endow JK, Dufour J, Zhu J, Inoue K (2014) Plastidic type I signal peptidase 1 is a redox-dependent thylakoidal processing peptidase. Plant J, 80(4): 592-603. [ジャーナルサイト] 緑川さんのUC Davisでの２報目の論文、チラコイド膜ルーメンへ輸送されるタンパク質の輸送シグナル切断酵素の性質を明らかにしました。

Enomoto, G., Nomura, R., Shimada, T., Ni-Ni-Win, Narikawa, R., Ikeuchi, M. (2014) Cyanobacteriochrome SesA is a diguanylate cyclase that induces cell aggregation in Thermosynechococcus. J. Biol. Chem., 289: 24801-9. [PubMed] [U_Tokyo news]

Narikawa, R., Enomoto, G., Win, N.N., Fushimi, K. and Ikeuchi, M. (2014) A new type of dual-Cys cyanobacteriochrome GAF domain found in cyanobacterium Acaryochloris marina, which has an unusual red/blue reversible photoconversion cycle. Biochemistry, 53: 5051-9. [PubMed] アカリオクロリスのゲノムから、新しいタイプのCys残基依存性光変換を示すシアノバクテリオクロムを発見しました。

Osanai, T, Oikawa, A, Iijima, H, Kuwahara, A, Asayama, M, Tanaka, K, Ikeuchi, M, Saito, K, Hirai MY. (2014) Metabolomic analysis reveals rewiring of Synechocystis sp. PCC 6803 primary metabolism by ntcA-overexpression. Environ. Microbiol. 10: 3304-3317. [PubMed]

Kiyota, H., Hirai, M.Y., Ikeuchi, M. (2014) NblA1/A2-dependent homeostasis of amino acid pools during nitrogen starvation in Synechocystis sp. PCC 6803. Metabolites, 4: 517-531. [PubMed] シアノバクテリアの窒素飢餓条件でのフィコビリソーム特異的な分解誘導の寄与をメタボローム的に評価しました。

K. Hori, F. Maruyama, T. Fujisawa, T. Togashi, N. Yamamoto, M. Seo, S. Sato, T. Yamada, H. Mori, N. Tajima, T. Moriyama, M. Ikeuchi, M. Watanabe, H. Wada, K. Kobayashi, M. Saito, T. Masuda, Y. Sasaki-Sekimoto, K. Mashiguchi, K. Awai, M. Shimojima, S. Masuda, M. Iwai, T. Nobusawa, T. N., S. Kondo, H. Saito, R. Sato, M. Murakawa, Y. Ihara, Y. Oshima-Yamada, K. Ohtaka, M. Satoh, K. Sonobe, M. Ishii, R. Ohtani, M. Kanamori-Sato, R. Honoki, D. Miyazaki, H. Mochizuki, J. Umetsu, K. Higashi, D. Shibata, Y. Kamiya, N. Sato, Y. Nakamura, S. Tabata, S. Ida, K. Kurokawa, and H. Ohta (2014) Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation. Nature Comm. 5: 3978. [PubMed] プレスリリース 藻類と陸上植物の中間的な存在である車軸藻植物門クレブソルミディウムのゲノムを解読し、藻類から陸上植物に至る遺伝子の進化過程を解明しました。東工大・太田先生らとのGCOE共同プロジェクトの成果です。

Kiyota, H., Okuda, Y., Ito, M., Hirai, M.Y., Ikeuchi, M. (2014) Engineering for photosynthetic limonene production by cyanobacteria from CO2. J. Biotechnol. 185: 1-7. [PubMed] シアノバクテリアに植物の酵素を導入して、揮発性イソプレノイドのリモネンの光合成による生産に成功しました。

Maeda, K., Narikawa, R., Ikeuchi, M. CugP is a novel ubiquitous non-GalU-type bacterial UDP-glucose pyrophosphorylase found in cyanobacteria. J. Bacteriol., 196: 2348-2354. [PubMed] シアノバクテリアに普遍的に存在する新規のUDPグルコース合成酵素を発見し、CugPと命名しました。

Watanabe, M., Semchonok, D.A., Webber-Birungi, M., Ehira, S., Kondo, K., Narikawa, R., Ohmori, M., Boekema, E.J. and Ikeuchi, M. Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria. Proc. Natl. Acad. Sci. U S A. 111: 2512-2517. [PubMed] プレスリリース アナベナの光化学系 I 複合体の４量体とフィコビリソームの超複合体を発見しました。Boekema教授との共同研究で、その超構造を解明しました。

２０１３年

Watanabe M., and Ikeuchi, M. (2013) Phycobilisome: architecture of a light-harvesting supercomplex. Photosynth. Res. 116: 265-276. [PubMed] フィコビリソームの構築のレヴュー：ロッドの「スケルトンモデル」を比較検討しました。

Osanai T, Oikawa A, Shirai T, Kuwahara A, Iijima H, Tanaka K, Ikeuchi M, Kondo A, Saito K, Hirai MY. (2014) Capillary electrophoresis-mass spectrometry reveals the distribution of carbon metabolites during nitrogen starvation in Synechocystis sp. PCC 6803. Environ Microbiol. 16: 512-524. [PubMed]

Escobar VF, Utesch T, Narikawa R, Ikeuchi M, Mroginski MA, Gärtner W, Hildebrandt P. (2013) Photoconversion mechanism of the second GAF domain of cyanobacteriochrome AnPixJ and the cofactor structure of its green-absorbing state. Biochemistry, 52: 4871-4880. [PubMed]

Gottlieb S.M., Kim P.W., Rockwell N.C., Hirose Y., Ikeuchi M., Lagarias J.C., Larsen D.S. (2013) Primary photodynamics of the green/red-absorbing photoswitching regulator of the chromatic adaptation E domain from Fremyella diplosiphon. Biochemistry, 52: 8198-8208. [PubMed]

Kuroi K, Tanaka K, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. (2013) Anomalous diffusion of TePixD and identification of the photoreaction product. Photochem Photobiol Sci. 12:1180-6. [PubMed]

Ohbayashi R, Watanabe S, Kanesaki Y, Narikawa R, Chibazakura T, Ikeuchi M, Yoshikawa H. (2013) DNA replication depends on photosynthetic electron transport in cyanobacteria. FEMS Microbiol Lett. 344: 138-44. [PubMed]

Osanai T, Kuwahara A, Iijima H, Toyooka K, Sato M, Tanaka K, Ikeuchi M, Saito K, Hirai MY. (2013) Pleiotropic effect of sigE over-expression on cell morphology, photosynthesis and hydrogen production in Synechocystis sp. PCC 6803. Plant J. 76: 456-465. [PubMed]

R. Nagao, T. Tomo, R. Narikawa, I. Enami, M. Ikeuchi (2013) Light-independent biosynthesis and assembly of the photosystem II complex in the diatom Chaetoceros gracilis. FEBS Lett. 587: 1340-1345. [PubMed]

Hirose, Y., Rockwell, N.C., Nishiyama, K., Narikawa, R., Ukaji, Y., Inomata, K., Lagarias, J.C., Ikeuchi, M. (2013) Green/red cyanobacteriochromes regulate complementary chromatic acclimation via a protochromic photocycle. Proc. Natl. Acad. Sci. U.S.A. 110: 4974-4979. [PubMed] ［プレスリリース］　光受容体の発色機構としてH+の脱着（プロトクロミックサイクル）を実証し、その分子背景を明らかにした。

Narikawa, R., Ishizuka, T., Muraki, N., Shiba, T., Kurisu, G. and Ikeuchi, M. (2013) Structures of cyanobacteriochromes from phototaxis regulators AnPixJ and TePixJ reveal general and specific photoconversion mechanism. Proc. Natl. Acad. Sci. U S A. 110: 918-23. [PubMed] ［プレスリリース］　当研究室で発見した新規光受容体シアノバクテリオクロム２種の結晶構造を解明しました。

２０１２年

Liauw P, Mashiba T, Kopczak M, Wiegand K, Muraki N, Kubota H, Kawano Y, Ikeuchi M, Hase T, Roegner M, Kurisu G. (2012) Cloning, expression, crystallization and preliminary X-ray studies of the ferredoxin-NAD(P)+ reductase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68: 1048-51. [PubMed]

Tanaka K, Nakasone Y, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. (2012) Time-resolved tracking of interprotein signal transduction: Synechocystis PixD-PixE complex as a sensor of light intensity. J Am Chem Soc. 134: 8336-9. [PubMed]

R. Nagao, T. Tomo, E. Noguchi, T. Suzuki, A. Okumura, R. Narikawa, I. Enami and M. Ikeuchi (2012) Proteases are associated with a minor fucoxanthin chlorophyll a/c-binding protein from the diatom, Chaetoceros gracilis. Biochim. Biophys. Acta 1817: 2110-2117. [PubMed]

Yoshimura H, Ikeuchi M, Ohomori M. (2012) Cell surface-associated proteins in the filamentous cyanobacterium Anabaena sp. strain PCC 7120. Microbes Environ. 27: 538-43. [PubMed]

Nowaczyk MM, Krause K, Mieseler M, Sczibilanski A, Ikeuchi M, Roegner M. (2012) Deletion of psbJ leads to accumulation of Psb27-Psb28 photosystem II complexes in Thermosynechococcus elongatus. Biochim Biophys Acta. 1817: 1339-45. [PubMed]

Enomoto, G., Hirose, Y., Narikawa, R. and Ikeuchi, M. (2012) Thiol-based photocycle of the blue and teal light-sensing cyanobacteriochrome Tlr1999. Biochemistry, 51: 3050-3058. [PubMed]

Kanesaki, Y., Shiwa, Y., Tajima, N., Suzuki, M., Watanabe, S., Sato, N., Ikeuchi, M., and Yoshikawa, H. (2012) Identification of substrain-specific mutations by massively parallel whole-genome resequencing of Synechocystis sp. PCC 6803. DNA Res. 19: 67-79. [PubMed]

Watanabe, M., Sato, M., Kondo, K., Narikawa, R. and Ikeuchi, M. (2012) Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa. Biochim. Biophys. Acta 1817: 1428-1435. [PubMed]

Midorikawa, T., Narikawa, R., and Ikeuchi, M. (2012) A deletion mutation in the spacing within the psaA core promoter enhances transcription in a cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 53: 164-172. [PubMed]

２０１１年

Narikawa, R., Suzuki, F., Yoshihara, S., Higashi, S., Watanabe, M. and Ikeuchi, M. (2011) Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 52: 2214-2224. [PubMed]

Noji, T., Suzuki, H., Gotoh, T., Iwai, M., Ikeuchi, M., Tomo, T., and Noguchi, T. (2011) Photosystem II gold nanoparticle conjugate as a nanodevice for the development of artificial light-driven water-splitting systems. J. Phys. Chem. Lett. 2: 2448-2452. [ACS Pub]

Fukushima, Y, Iwaki M, Narikawa R, Ikeuchi M, Tomita Y, Itoh S. (2011) Photoconversion mechanism of a green/red photosensory cyanobacteriochrome AnPixJ: time-resolved optical spectroscopy and FTIR analysis of the AnPixJ-GAF2 domain. Biochemistry 50: 6328-39. [PubMed]

Tajima, N., Sato, S., Maruyama, F., Kaneko, T., Sasaki, N.V., Kurokawa, K., Ohta, H., Kanesaki, Y., Yoshikawa, H., Tabata, S., Ikeuchi, M., and Sato, N. (2011) Genomic structure of the cyanobacterium Synechocystis sp. PCC 6803 Strain GT-S. DNA Res. 18: 393-9. [PubMed]

Tanaka K, Nakasone Y, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. (2011) A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change. FEBS Lett. 585: 786-90. [PubMed]

Tanaka K, Nakasone Y, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. (2011) Light-induced conformational change and transient dissociation reaction of the BLUF photoreceptor Synechocystis PixD (Slr1694). J Mol Biol. 409: 773-85. [PubMed]

Osanai, T., Oikawa, A., Azuma, M., Tanaka, K., Saito, K., Hirai, M.Y., and Ikeuchi, M. (2011) Genetic engineering of the group 2 sigma factor SigE widely activates the expressions of sugar catabolic genes in Synechocystis sp. PCC 6803. J Biol Chem. 286: 30962-30971. [PubMed]

Yoshimura, H., Kotake, T., Aohara, T., Tsumuraya, Y., Ikeuchi, M. and Ohmori, M. (2012) The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp. strain HK-01 in NaCl tolerance. J. Appl. Phycol. 24: 237-243. [SpringerLink]

Kawano, Y., Saotome, T., Ochiai, Y., Katayama, M., Narikawa, R. and Ikeuchi, M. Cellulose accumulation and a cellulose synthase gene are responsible for cell aggregation in the cyanobacterium Thermosynechococcus vulcanus RKN. Plant Cell Physiol. 52: 957-66. [PubMed]

Toyooka T, Tanaka K, Okajima K, Ikeuchi M, Tokutomi S, and Terazima M. Macromolecular crowding effects on reactions of TePixD (Tll0078). Photochem Photobiol. 87: 584-589. [PubMed]

Kawakami, K., Umena, Y., Iwai, M., Kawabata, Y., Ikeuchi, M., Kamiya, N. and Shen, J.-R. (2011) Roles of PsbI and PsbM in photosystem II dimer formation and stability studied by deletion mutagenesis and X-ray crystallography. Biochim. Biophys. Acta, 1807: 319-325. [PubMed]

Ishizuka, T., Kamiya, A., Suzuki, H., Narikawa, R., Noguchi, T., Kohchi, T., Inomata, K., and Ikeuchi, M. (2011) The cyanobacteriochrome, TePixJ, isomerizes its own chromophore by converting phycocyanobilin to phycoviolobilin. Biochemistry, 50: 953-961. [PubMed]

Watanabe, M., Kubota, H., Wada, H., Narikawa, R. and Ikeuchi, M. (2011) Novel supercomplex organization of Photosystem I in Anabaena and Cyanophora paradoxa. Plant Cell Physiol. 52: 162-168. [PubMed]

２０１０年

Hirose, Y., Narikawa, R., Katayama, M. and Ikeuchi, M. (2010) Cyanobacteriochrome CcaS regulates phycoerythrin accumulation in Nostoc punctiforme, a group II chromatic adapter. Proc. Natl. Acad. Sci. U.S.A. 107: 8854-8859. [PubMed]

Yoshimura, H., Kaneko, Y., Ehira, S., Yoshihara, S., Ikeuchi, M. and Ohmori, M. (2010) CccS and CccP are involved in construction of cell surface components in the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Cell Physiol. 51: 1163-72. [PubMed]

Nagao, R., Suzuki, T., Okumura, A., Niikura, A., Iwai, M., Dohmae, N., Tomo, T., Shen, J.R., Ikeuchi, M. and Enami, I. (2010) Topological analysis of the extrinsic PsbO, PsbP and PsbQ proteins in a green algal Photosystem II complex by crosslinking with a water-soluble carbodiimide. Plant Cell Physiol. 51: 718-727. [PubMed]

Fujisawa T, Narikawa R, Okamoto S, Ehira S, Yoshimura H, Suzuki I, Masuda T, Mochimaru M, Takaichi S, Awai K, Sekine M, Horikawa H, Yashiro I, Omata S, Takarada H, Katano Y, Kosugi H, Tanikawa S, Ohmori K, Sato N, Ikeuchi M, Fujita N, Ohmori M. (2010) Genomic structure of an economically important cyanobacterium, Arthrospira (Spirulina) platensis NIES-39. DNA Res. 17: 85-103. [PubMed]

Iwai M, Suzuki T, Kamiyama A, Sakurai I, Dohmae N, Inoue Y, Ikeuchi M. (2010) PsbK subunit is required for the stable assembly and stability of other small subunits in the Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiol. 51: 554-560. [PubMed]

Takasaka K, Iwai M, Umena Y, Kawakami K, Ohmori Y, Ikeuchi M, Takahashi Y, Kamiya N, Shen JR. (2010) Structural and functional studies on Ycf12 (Psb30) and PsbZ-deletion mutants from a thermophilic cyanobacterium. Biochim Biophys Acta. 1797:278-84. [PubMed]

Nagao R, Tomo T, Noguchi E, Nakajima S, Suzuki T, Okumura A, Kashino Y, Mimuro M, Ikeuchi M, Enami I. (2010) Purification and characterization of a stable oxygen-evolving Photosystem II complex from a marine centric diatom, Chaetoceros gracilis. Biochim Biophys Acta. 1797: 160-6. [PubMed]

Allakhverdiev, S.I., Tomo, T., Shimada, Y., Kindo, H., Nagao, R., Klimov, V.V., and Mimuro, M. (2010) Redox potential of pheophytin a in photosystem II of two cyanobacteria having the different special pair chlorophylls. Proc. Natl. Acad. Sci. U S A. 107: 3924-3929. [PubMed]

Shibamoto, T., Kato, Y., Nagao, R., Yamazaki, T., Tomo, T., Watanabe, T. (2010) Species-dependence of the redox potential of the primary quinone electron acceptor Q_A in photosystem II verified by spectroelectrochemistry. FEBS Lett. 584: 1526-30. [PubMed]

２００９年

Cormann KU, Ikeuchi M, Rogner M, Nowaczyk MM, Stoll R. (2009) Sequence-specific (1)H, (13)C, and (15)N backbone assignment of Psb27 from Synechocystis PCC 6803. Biomol NMR Assign. 3: 247-249. [PubMed]

Nagao R, Tomo T, Noguchi E, Nakajima S, Suzuki T, Okumura A, Kashino Y, Mimuro M, Ikeuchi M, Enami I.(2009) Purification and characterization of a stable oxygen-evolving Photosystem II complex from a marine centric diatom, Chaetoceros gracilis. Biochim Biophys Acta. 1797: 160-1663. [PubMed]

Cormann KU, Bangert JA, Ikeuchi M, Rogner M, Stoll R, Nowaczyk MM. (2009) Structure of Psb27 in solution: implications for transient binding to photosystem II during biogenesis and repair. Biochemistry. 48: 8768-70. [PubMed]

Midorikawa T, Matsumoto K, Narikawa R, Ikeuchi M. (2009) An Rrf2-type transcriptional regulator is required for expression of psaAB genes in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol. 151: 882-92. [PubMed]

Watanabe M, Iwai M, Narikawa R, Ikeuchi M. (2009) Is the photosystem II complex a monomer or a dimer? Plant Cell Physiol. 50: 1674-80. [PubMed]

Tanaka K, Nakasone Y, Okajima K, Ikeuchi M, Tokutomi S, Terazima M. (2009) Oligomeric-state-dependent conformational change of the BLUF protein TePixD (Tll0078). J Mol Biol. 386: 1290-300. [PubMed]

Shibata Y, Murai Y, Satoh Y, Fukushima Y, Okajima K, Ikeuchi M, Itoh S. (2009) Acceleration of electron-transfer-induced fluorescence quenching upon conversion to the signaling state in the blue-light receptor, TePixD, from Thermosynechococcus elongatus. J Phys Chem B. 113: 8192-8. [PubMed]

Osanai T, Imashimizu M, Seki A, Sato S, Tabata S, Imamura S, Asayama M, Ikeuchi M, Tanaka K. (2009) ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803. Proc Natl Acad Sci U S A. 106: 6860-5. [PubMed]

Narikawa R, Muraki N, Shiba T, Ikeuchi M, Kurisu G. (2009) Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65: 159-62. [PubMed]

２００８年

Kondo K, Mullineaux CW, Ikeuchi M. (2009) Distinct roles of CpcG1-phycobilisome and CpcG2-phycobilisome in state transitions in a cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res. 99: 217-25. [PubMed]

Myouga F, Hosoda C, Umezawa T, Iizumi H, Kuromori T, Motohashi R, Shono Y, Nagata N, Ikeuchi M, Shinozaki K. (2008) A heterocomplex of iron superoxide dismutases defends chloroplast nucleoids against oxidative stress and is essential for chloroplast development in Arabidopsis. Plant Cell. 20: 3148-62. [PubMed]

Nagai H, Fukushima Y, Okajima K, Ikeuchi M, Mino H. (2008) Formation of interacting spins on flavosemiquinone and tyrosine radical in photoreaction of a blue light sensor BLUF protein TePixD. Biochemistry. 47: 12574-82. [PubMed]

Narikawa R, Kohchi T, Ikeuchi M.(2008) Characterization of the photoactive GAF domain of the CikA homolog (SyCikA, Slr1969) of the cyanobacterium Synechocystis sp. PCC 6803. Photochem Photobiol Sci. 7: 1253-9. [PubMed]

Ikeuchi M, Ishizuka T. (2008) Cyanobacteriochromes: a new superfamily of tetrapyrrole-binding photoreceptors in cyanobacteria. Photochem Photobiol Sci. 7: 1159-67. [PubMed]

Iwai M, Maoka T, Ikeuchi M, Takaichi S. (2008) 2,2'-beta-hydroxylase (CrtG) is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2'-fucoside in Thermosynechococcus elongatus strain BP-1. Plant Cell Physiol. 49: 1678-87. [PubMed]

Sato S, Ikeuchi M, Nakamoto H. (2008) Expression and function of a groEL paralog in the thermophilic cyanobacterium Thermosynechococcus elongatus under heat and cold stress. FEBS Lett. 582: 3389-95. [PubMed]

Suzuki H, Okajima K, Ikeuchi M, Noguchi T. (2008) LOV-like flavin-Cys adduct formation by introducing a Cys residue in the BLUF domain of TePixD. J Am Chem Soc. 130: 12884-5. [PubMed]

Hirose Y, Shimada T, Narikawa R, Katayama M, Ikeuchi M. (2008) Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein. Proc Natl Acad Sci U S A. 105: 9528-33. [PubMed]

Narikawa R, Fukushima Y, Ishizuka T, Itoh S, Ikeuchi M. (2008) A novel photoactive GAF domain of cyanobacteriochrome AnPixJ that shows reversible green/red photoconversion. J Mol Biol. 380: 844-55. [PubMed]

Higo A, Ikeuchi M, Ohmori M. (2008) cAMP regulates respiration and oxidative stress during rehydration in Anabaena sp. PCC 7120. FEBS Lett. 582: 1883-8. [PubMed]

Henmi T, Iwai M, Ikeuchi M, Kawakami K, Shen JR, Kamiya N. (2008) X-ray crystallographic and biochemical characterizations of a mutant photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation. J Synchrotron Radiat. 15: 304-7. [PubMed]

Osanai T, Ikeuchi M, Tanaka K. (2008) Group 2 sigma factors in cyanobacteria. Physiol Plant. 133: 490-506. [PubMed]

Hishiya S, Hatakeyama W, Mizota Y, Hosoya-Matsuda N, Motohashi K, Ikeuchi M, Hisabori T. (2008) Binary reducing equivalent pathways using NADPH-thioredoxin reductase and ferredoxin-thioredoxin reductase in the cyanobacterium Synechocystis sp. strain PCC 6803. Plant Cell Physiol. 49: 11-8. [PubMed]

２００７年

Kondo, K., Katayama, M. and Ikeuchi, M., (2007) Specific interaction between CpcG2-phycobilisome and photosystem I. in PS2007 Proceedings: 14th International Congress on Photosynthesis.

Kawakami, K., Kawabata, Y., Henmi, T., Iwai, M., Suematsu, T., Ikeuchi, M., Kamiya, N. and Shen, J-R. (2007) Crystallization and crystal structure analysis of a mutant photosystem II complex lacking PsbI from Thermosynechococcus vulcanus. in PS2007 Proceedings: 14th International Congress on Photosynthesis.

Fukushima, Y., Murai, Y., Okajima, K., Ikeuchi, M. and Itoh, S. (2007) Photoreactions of Tyr8- and Gln50-mutated BLUF domains of the PixD protein of Thermosynechococcus elongatus BP-1: Photoconversion at low temperature without Tyr-8. Biochemistry, 47: 660-669. [PubMed]

Ishino Y, Okada H, Ikeuchi M, Taniguchi H. (2007) Mass spectrometry-based prokaryote gene annotation. Proteomics, 7: 4053-65.

Higo, A., Suzuki T, Ikeuchi M, and Ohmori M. (2007) Dynamic transcriptional changes in response to rehydration in Anabaena sp. PCC 7120. Microbiology, 153: 3685-94.

Iwai, M., Suzuki, T., Dohmae, N., Inoue, Y. and Ikeuchi, M. (2007) Absence of the PsbZ subunits prevents association of the PsbK and Ycf12 with the photosystem II in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiol. 48: 1758--63.

Kawakami, K., Iwai, M., Ikeuchi, M., Kamiya, N. and Shen, J-R. (2007) Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography. FEBS Lett. 581: 4983--4987.

Ishizuka, T., Narikawa, R., Kohchi, T., Katayama, M. and Ikeuchi, M. (2007) Cyanobacteriochrome TePixJ of Thermosynechococcus elongatus harbors phycoviolobilin as a chromophore. Plant Cell Physiol. 48: 1385--1390.

TePixJ を酸性尿素で変性させ、スペクトル解析を行ってその発色団の同定を試みた。その結果TePixJの発色団はフィコビオロビリンである事が示唆された。フィトクロムの発色団はフィトクロモビリン、フィコシアノビリン、ビリベルジンのいずれかであるが、フィコビオロビリンは共役二重結合系がこれらの発色団より短いので、より短波長の光を吸収する事ができる。またフィコシアノビリン産生大腸菌で発現させたTePixJの発色団がフィコビオロビリンであるという結果を得た。このことからフィコビオロビリンはTePixJ自身がフィコシアノビリンを異性化することで供給されていることが示唆された。TePixJは光変換活性、発色団を結合するリアーゼ活性、異性化活性といった多機能性を保持した光受容体であることが分かった。

Kondo, K., Ochiai, Y., Katayama, M. and Ikeuchi, M. (2007) The membrane-associated CpcG2-phycobilisome in Synechocystis; a new Photosystem I antenna. Plant Physiol. 144: 1200--10. [PubMed]

CpcG2が形成するフィコビリソームがチラコイド膜により強く相互作用していること、PSIのアンテナとして機能していることが示唆され、CpcG2と同様にC末端に疎水性領域を有するCpcGが広く保存されていることが分かった。

Takahashi, R., Okajima, K., Suzuki, H., Nakamura, H., Ikeuchi, M. and Noguchi, T. (2007) FTIR study on the hydrogen-bond structure of a key tyrosine residue in the flavin-binding blue light sensor TePixD from Thermosynechococcus elongatus. Biochemistry 46: 6459-6467. [PubMed]

Ozaki, H., Ikeuchi, M., Ogawa, T., Fukuzawa, H. and Sonoike, K. (2007) Large scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp. PCC 6803: Identification of the factors involved in the modulation of photosystem stoichiometry. Plant Cell Physiol. 48: 451-458. [PubMed]

Fukushima, Y., Okajima, K., Ikeuchi, M. and Itoh, S. (2007) Two intermediate states I and J trapped at low temperature in the photocycles of two BLUF domain proteins of cyanobacteria Synechocystis sp. PCC 6803 and Thermosynechococcus elongatus BP-1. Photochem Photobiol. 83: 112-121. [PubMed]

Suzuki, T., Yoshimura, H., Ehira, S., Ikeuchi, M., and Ohmori, M. (2007) AnCrpA, a cAMP receptor protein, regulates nif-related gene expression in the cyanobacterium Anabaena sp. strain PCC 7120 grown with nitrate. FEBS Lett. 581: 21-8.

２００６年

Narikawa, R., Zikihara, K., Okajima, K., Ochiai, Y., Katayama, M., Shichida, Y., Tokutomi, S. and Ikeuchi, M. Three putative photosensory flavin-PAS domains with distinct biochemical properties from the filamentous cyanobacterium Anabaena sp. PCC 7120. Photochem. Photobiol. 82: 1627-33.

成川君のPASドメイン解析のひとつ、フラビンPAS(Alr3170, All2875, Alr1229, AvAll2875)の生化学、分光解析の論文である。大阪府大徳富先生らと共同で、シアノバクテリアでは初のフォトトロピン型FMN結合光受容体を同定した。

Seki, A., Nakano, T., Takahashi, H., Matsumoto, K., Ikeuchi, M., and Tanaka, K. (2006) Light-responsive transcriptional regulation of the suf promoters involved in Fe-S cluster biogenesis in a cyanobacterium Synechocystis sp. PCC 6803. FEBS Lett. 580: 5044-5048.

分生研・田中先生と関さんの光応答型sufオペロン転写の研究に協力しました。内容としては、sufオペロンのプロモータの同定と好熱シアノバクテリアのSufRによるin vitro転写への関与を示した。

Okajima, K. Fukushima, Y., Suzuki, H., Kita, A., Ochiai, Y., Katayama, M., Shibata, Y., Miki, K., Noguchi, T., Itoh, S. and Ikeuchi, M. (2006) Fate determination of the flavin photoreceptions in the cyanobacterial blue light receptor TePixD (Tll0078). J. Mol. Biol. 363: 10-18.

BLUF型フラビン結合センサーTePixDの部位特異変異解析により、フラビンの光反応に大きな影響を与える残基を同定した。Gln50をAsnに置換したタンパク質はフラビンの長波長シフトを起こさず、代わりにトリプレットが生成し、効率は低いがフラビンの光還元が引き続いて起こった。同様の現象は、Gln50と相互作用しているTyr8をPheもしくはAlaに置換した変異タンパク質でも起きた。しかし、 Gln50をAlaに置換したものではトリプレットも生成せず、光還元も長波長シフトも示さなかった。一方、Asn32をAlaに置換した変異タンパク質はFT-IR解析で明・暗ともにC(4)=Oと水素結合していることが示された。以上の結果は、Gln50のアミド側鎖がフラビンのC(4)＝Oと N(5)に正しく配向することがBLUF型光反応に重要であることを示しているとともに、この配向が崩れると、フォトトロピン型の光反応に似た挙動をとりやすいことを示している。論文の図をHPトップページに追加。

Ishizuka, T., Shimada, T., Okajima, K., Yoshihara, S., Ochiai, Y., Katayama M. and Ikeuchi, M. (2006) Characterization of cyanobacteriochrome TePixJ from a thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1. Plant Cell Physiol. 47: 1251-1261.

ついにシアノバクテリアの新しい光受容体の大量精製に成功しました。これは好熱性シアノバクテリアのPixJのGAFドメインをSynechocystisに発現させたものである。論文の図をHPトップページに追加。

Higo, A., Katoh, H., Ohmori, K., Ikeuchi, M. and Ohmori, M. (2006) The role of a gene cluster for trehalose metabolism in dehydration tolerance of the filamentous cyanobacterium Anabaena sp. PCC 7120. Microbiology 152: 979-987.

糸状性シアノバクテリアAnabaena 7120における乾燥によるトレハロース合成、代謝遺伝子の誘導のその役割を明らかにした。

Kobayashi, M., Katoh, H. and Ikeuchi, M. (2006) Mutations in a putative chloride efflux transporter gene suppress the chloride requirement of the photosystem II in psbV-disruptant (or cytochrome c550 deficient mutant). Plant Cell Physiol. 47: 799-804.

クロライドイオンを要求するpsbV変異体から必要としなくなったサプレッサー変異株を単離同定した。この変異遺伝子は未知の輸送体をコードしていた。これは、哺乳動物のメラニン合成にかかわる未知の輸送体様遺伝子、大腸菌などの亜砒酸排出ポンプに有意な相同性があり、クロライド排出ポンプの可能性が推定された。

Yoshimura, H., Ikeuchi, M. and Ohmori, M. (2006) Up-regulated gene expression during dehydration in terrestrial cyanobacterium Nostoc sp. HK-01. Microbe Environ., 21: 129-133.

Iwai, M., Katayama, M. and Ikeuchi, M. (2006) Absence of the psbH gene product destabilizes Photosystem II complex: the lack of PSII-X protein in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Photosynth. Res. 87: 313-322.

好熱性シアノバクテリアのpsbH破壊株の解析より、PSII-Xタンパク質の消失、光化学系２複合体の二量体化の阻害を見出した。

２００５年

Kobayashi, M., Okada, K. and Ikeuchi, M. (2005) A suppressor mutation in a-phycocyanin gene in light/glucose-sensitive phenotype of psbK-disruptant of the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 46: 1561-1567.

psbK変異株から単離したサプレッサー変異として、cpcA遺伝子内の大きなinverted repeat形成変異を同定した。変異の起こるしくみ、psbK変異の表現型の再確認は重要な知見である。

Ohta H, Shibata Y, Haseyama Y, Yoshino Y, Suzuki T, Kagasawa T, Kamei A, Ikeuchi M, Enami I. (2005) Identification of genes expressed in response to acid stress in Synechocystis sp. PCC 6803 using DNA microarrays. Photosynth. Res. 84: 225-230.

Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M. Higashi, S., Watanabe, M., Sato, S., Tabata, S., Shibata, Y., Itoh, S. and Ikeuchi, M. (2005) Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria. Journal of Biochemistry, 137: 741-750.

新型フラビン(FAD)結合型青色センサータンパク質Tll0078とSlr1694の比較解析論文。好熱菌由来のTll0078の方がはるかに熱安定性が高いこと、水溶性ジチオナイトに還元されにくいこと、長時間光照射に応じて可逆的な構造変化を生じること(名大、福島、伊藤先生との共同)、Synechocystisの野生株と破壊株の走光性の解析より正の走光性に必須であること(PixDと命名、基生研大型スペクトログラフ、東、渡辺先生との共同)、酵母２ハイブリッド解析（かずさDNA研、佐藤・田畑先生との共同）により相互作用する因子としてSlr1693(PixEと命名)を同定。

Zhang, Battchikova, N., Paakkarinen, V., Katoh, H., Iwai, M., Ikeuchi, M., Pakrasi, H.B., Ogawa, T. and Aro, E.-V. Isolation, subunit composition and interaction of the NDH-1 complexes from Thermosynechococcus elongatus BP-1. Biochemical Journal, 390: 513-520.

好熱性シアノバクテリアを用いて、NADPH dehydrogenase複合体を効率よく単離できる系が確立できました。（フィンランド、Aro先生グループ、中国、小川先生との共同研究）

Kondo, K., Geng, X., Katayama, M. and Ikeuchi, M. (2005) Distinct roles of CpcG1 and CpcG2 in phycobilisome assembly in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthesis Research, 84: 269-273.

シアノバクテリアの光合成アンテナ色素複合体であるフィコビリソームの新サブユニット (CpcG2)を見いだした。これは、従来知られていたロッドコアリンカーCpcG1のパラログで、従来型とは異なる新規のフィコビリソームを形成していた。

Kita, A., Okajima, K., Morimoto, Ikeuchi, M. and Miki, K. (2005) Structure of a cyanobacterial BLUF protein, Tll0078, containing a novel FAD-binding blue light sensor domain. Journal of Molecular Biology 349: 1-9.

京大、喜田・三木先生グループとの共同で、ついに新型フラビン(FAD)結合型青色センサータンパク質Tll0078の結晶構造を2.0Aの解像度で決定。さらに、イソアロキサジン環と相互作用する重要と思われる残基Asn31、Asn32、Gln50をそれぞれAlaに置換した変異タンパク質を作製し、Gln50が光応答にもっとも重要な残基であることを証明しました。

Fukushima, Y., Okajima, K., Shibata, Y., Ikeuchi, M. and Itoh, S. (2005) Primary intermediate in photocycle of a blue light sensory BLUF FAD-protein Tll0078 of Thermosynechococcus elongatus BP-1. Biochemistry 44: 5149-5158.

名大、福島、伊藤先生グループとの共同研究。液体ヘリウム温度(10K)で光照射することで、新型フラビン (FAD)結合型青色センサータンパク質の初めての光変換中間体を同定。

２００４年

Iwai, M., Katoh, H., Katayama, M. and Ikeuchi, M. (2004) PSII-Tc protein plays an important role in dimerization of Photosystem II. Plant & Cell Physiology 45: 1809-1816.

Yoshihara, S., Katayama, M., Geng, X. and Ikeuchi, M. (2004) Cyanobacterial phytochrome-like PixJ1 holoprotein shows novel reversible photoconversion between blue- and green-absorbing forms. Plant & Cell Physiology 45: 1729-1737.

シアノバクテリアの正の走光性の光受容体PixJ1をSynechocystis細胞より部分精製した。得られたPixJ1は共有結合で開環テトラピロールを結合しており、425-435 nm吸収型(Pb)と535 nm吸収型(Pg)の間で可逆的な光変換を示した。PixJ1はフィトクロムと相同性を示す2個のGAFドメインをもつが、その後半のGAFドメインの Cys-HisモチーフをAla-Alaに変えた変異体タンパク質は色素結合を示さなかった。このように、PixJ1は植物フィトクロムと似てはいるが、独自の光受容体であり、シアノバクテリアに広く分布しているので、その関連分子を含めて、「シアノバクテリオクロム」と命名した。

Yoshihara, S. and Ikeuchi, M. (2004) Phototactic motility in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Photochemistry & Photobiology Science 3: 512-8. [PubMed]

Narikawa, R., Okamoto, S., Ikeuchi, M. and Ohmori, M. (2004) Molecular evolution of PAS domain-containing proteins of filamentous cyanobacteria through domain shuffling and domain duplication. DNA Research 11: 69-81. [PDF download]

Sato, N., Ohmori, M., Ikeuchi, M., Tashiro, K., Wolk, P.C., Kaneko, T., Okada, K., Tsuzuki, M., Ehira, S., Katoh, H., Okamoto, S., Yoshimura, H., Fujisawa, T., Kamei, A., Yoshihara, S., Narikawa, R., Hamano, T., Tabata, S., Kuhara, S. (2004) Use of segment-based microarray in the analysis of global gene expression in response to various environmental stresses in the cyanobacterium Anabaena sp. PCC 7120. Journal of General & Applied Microbiology 50: 1-8. [PubMed]

Kobayashi, M., Isizuka, T., Katayama, M., Kanehisa, M., Bhattacharyya-Pakrasi, M., Pakrasi, H.B. and Ikeuchi , M. (2004) Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 45: 290-299. [PubMed]

Iwai, M., Katoh, H., Katayama, M. and Ikeuchi, M. (2004) Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1. Plant & Cell Physiology 45: 171-175. [PubMed]

２００３年

Itaya, M.,, Fujita, K., Ikeuchi, M., Koizumi, M. and Tsuge, K. (2003) Stable positional cloning of long continuous DNA in the Bacillus subtilis genome vector. Journal of Biochemistry 134: 513-519. [PubMed]

Narikawa, R., Okamoto, S., Ikeuchi, M. and Ohmori, M. (2003) Newly identified motifs within PAS domains of filamentous cyanobacteria. Genome Informatics 14: 444-445. [PDF download]

Hihara, Y., Sonoike, K., Kanehisa, M. and Ikeuchi, M. (2003) DNA microarray analysis of redox responsive genes in the genome of the cyanobacterium Synechocystis sp. strain PCC 6803. Journal of Bacteriology 185: 1719-1725. [PubMed]

Kamei, A, Yoshihara, S., Yuasa, T., Geng, X. and Ikeuchi, M. (2003) Biochemical and functional characterization of a eukaryotic-type protein kinase, SpkB, in the cyanobacterium, Synechocystis sp. PCC 6803. Current Microbiology 46: 296-301. [PubMed]

２００２年

Kashino, Y., Koike, H., Yoshio, M., Egashira, H., Ikeuchi, M., Pakrasi, H.B. and Satoh, K. (2002) Low-molecular-mass polypeptide components of a photosystem II preparation from the thermophilic cyanobacterium Thermosynechococcus vulcanus. Plant & Cell Physiology 43: 1366-1373. [PubMed]

Nakamura, Y., Kaneko, T., Sato, S., Ikeuchi, M., Katoh, H., Sasamoto, S., Watanabe, A., Iriguchi, M., Kawashima, K., Kimura, T., Kishida, Y., Kiyokawa, C., Kohara, M., Matsumoto, M., Matsuno, A., Nakazaki, N., Shimpo, S., Sugimoto, M., Takeuchi, C., Yamada, M. and Tabata, S. (2002) Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. DNA Research 9: 123-130 [PDF download]

Kamei, A., Yuasa, T., Geng, X. and Ikeuchi, M. (2002) Biochemical examination of the potential eukaryotic-type protein kinase genes in the complete genome of the unicellular cyanobacterium Synechocystis sp. PCC 6803. DNA Research 9: 71-78. [PubMed]

Yoshihara, S., Geng, X. and Ikeuchi, M. (2002) pilG gene cluster and split pilL genes involved in pilus biogenesis, motility and genetic transformation in the cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 43: 513-521. [PubMed]

Yoshimura, H., Yoshihara, S., Okamoto, S., Ikeuchi, M. and Ohmori, M. (2002) A cAMP receptor protein, SYCRP1, is responsible for the cell motility of Synechocystis sp. PCC 6803. Plant & Cell Physiology, 43: 460-463. [PubMed]

Furumichi, M., Sato, Y., Omata, T., Ikeuchi, M. and Kanehisa, M. (2002) SYORF: Community annotation of cyanobacteria genes. Genome Informatics 13: 402-403.

２００１年

Ohmori, M., Ikeuchi, M., Sato, N., Wolk, P., Kaneko, T., Ogawa, T., Kanehisa, M., Goto, S., Kawashima, S., Okamoto, S., Yoshimura, H., Katoh, H., Fujisawa, T., Ehira, S., Kamei, A., Yoshihara, S., Narikawa, R. and Tabata, S. (2001) Characterization of genes encoding multi-domain proteins in the genome of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Research, 8: 271-284. [PDF download]

Ikeuchi, M. and Tabata, S. (2001) Synechocystis sp. PCC 6803: a useful tool in the study of the genetics of cyanobacteria. Photosynthesis Research, 70: 73-83. [PDF download]

Kamei, A., Hihara, Y., Yoshihara, S., Geng, X., Kanehisa, M. and Ikuechi, M. (2001) Functional Analysis of lexA-like gene, sll1626 in Synechocystis sp. PCC 6803 using DNA microarray. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S41-013, CSIRO Publishing: Melbourne.

Yoshihara, S., Kobayashi, R., Geng, X. and Ikeuchi, M. (2001) A novel gene involved in phototactic motility of the unicellular cyanobacterium Synechcocystis sp. PCC 6803. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S24-020, CSIRO Publishing: Melbourne.

Kobayashi, M., Katoh, H. and Ikeuchi, M. (2001) Analysis of mutations to suppress the chloride requirement of the psbV-disruptant of the cyanobacterium Synechocystis sp. PCC 6803. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S25-009, CSIRO Publishing: Melbourne.

Iwai, M., Katoh, H. and Ikeuchi, M. (2001) Functional analysis of PSII-T protein of photosystem II complex from the thermophilic cyanobacterium, Thermosynechococcus (formerly Synechococcus) elongatus BP-1. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S22-031, CSIRO Publishing: Melbourne.

Katoh, H. and Ikeuchi, M. (2001) Characterization of PSII-I or PSII-K protein-depleted photosystem II from Thermosynechococcus elongatus strain BP-1. PSII-I protein plays an essential role in dimerization of photosystem II. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S5-50, CSIRO Publishing: Melbourne.

Hihara, Y., Hiyama, T., Kanehisa, M. and Ikeuchi, M. (2001) DNA microarray analysis of Synechocystis sp. PCC 6803 cells under PSII and PSI light conditions. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S41-015, CSIRO Publishing: Melbourne.

Aiba, H., Kamei, K., Ikeuchi, M., Ogawa, T., Fukuzawa, H., and Sonoike, K. (2001) Analysis of a cyanobacterium Synechocystis sp. PCC 6803 genome by application of chlorophyll fluorescence video-imaging system. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S41-010, CSIRO Publishing: Melbourne.

Fukuzawa, H., Miura, K., Matsui, Y., Irie, T., Ogawa, T., Ikeuchi, M., Kaneko, T. and Tabata, S. (2001) Isolation of high-CO2 requiring mutants through the systematic gene disruption system in a cyanobacterium, Synechocystis sp. PCC 6803. in PS2001 Proceedings: 12th International Congress on Photosynthesis. S41-024, CSIRO Publishing: Melbourne.

Katoh, H., Itoh, S., Shen, J.R. and Ikeuchi, M. (2001) Functional analysis of psbV and a novel c-type cytochrome gene psbV2 of the thermophilic cyanobacterium Thermosynechococcus elongatus. Plant & Cell Physiology, 42: 599-607. [PubMed]

Hihara, Y., Kamei, A., Kanehisa, M., Kaplan, A. and Ikeuchi, M. (2001) DNA microarray analysis of cyanobacterial gene expression during acclimation to high light. Plant Cell 13: 793-806. [PubMed] [PDF download]

Sonoike, K. Hihara, Y. and Ikeuchi, M. (2001) Physiological significance of the regulation of photosystem stoichiometry upon high light acclimation of Synechocystis sp. PCC 6803. Plant & Cell Physiology, 42: 379-384. [PubMed]

Kamei, A., Yuasa, T., Orikawa, K., Geng, X.X. and Ikeuchi, M. (2001) A eukaryotic-type protein kinase SpkA is required for the normal motility of the unicellular cyanobacterium, Synechocystis sp. PCC 6803. Journal of Bacteriology, 183: 1505-1510. [PubMed][PDF download]

Katoh, H. and Ikeuchi, M. (2001) Targeted disruption of psbX and biochemical characterization of Photosystem II complex in the thermophilic cyanobacterium Thermosynechococcus elongatus. Plant & Cell Physiology, 42: 179-188. [PubMed]

Satoh, S., M. Ikeuchi, Mimuro, M. and Tanaka, A. (2001) Chlorophyll b in expressed in cyanobacteria functions as a light-harvesting antenna in photosystem I through flexibility of the proteins. Journal of Biological Chemistry, 276: 4293-4297. [PubMed][PDF download]

Yoshihara, S., Geng, X.X., Okamoto, S., Yura, K., Murata, T., Go, M., Ohmori, M. and Ikeuchi, M. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology, 42: 63-73. [PubMed]

２０００年

Yoshihara, S., Suzuki, F., Fujita, H., Geng, X.X. and Ikeuchi, M. (2000) Novel putative photoreceptor and regulatory genes required for the positive phototactic movement of the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology, 41: 1299-1304. [PubMed]

Uchiyama, I., Miwa, T., Nishide, H., Suzuki, I., Omata, T., Ikeuchi, M., Murata, N. and Kanehisa, M. (2000) Data submission system for cyanobacterial DNA chip consortium. Genome Informatics 11: 235-236.

Okuji, Y.K., Hihara, Y., Kamei, A., Ikeuchi, M., Suzuki, I., Murata, N. and Kanehisa, M. (2000) Gene expression analysis of orthologs in Synechocystis sp. with operons in Escherichia coli. Genome Informatics 11: 233-234.

１９９９年

Okamoto, S., Ikeuchi, M. and Ohmori, M. (1999) Experimental analysis of recently transposed insertion sequences in the cyanobacterium Synechocystis sp. PCC 6803. DNA Research, 6: 265-273. [PDF download]

Umeda, Y., Hirano, A., Ishibashi, M., Akiyama, H., Onizuka, T., Ikeuchi, M. and Inoue, Y. (1999) Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664: Implication of a novel set of cellulose synthase genes. DNA Research, 6: 109-115. [PDF download]

Martinson, T.A., M. Ikeuchi, M. and Plumley, F.G. (1998) Oxygen-evolving diatom thylakoid membranes. Biochim. Biophys. Acta 1409: 72-86.

Hihara, Y., Sonoike, K. and Ikeuchi, M. (1998) A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis sp. PCC 6803 in response to high light. Plant Physiology, 117: 1205-1216. [PubMed][PDF download]

Hihara, Y. and Ikeuchi, M. (1998) Toward the elucidation of physiological significance of pmgA-mediated high-light acclimation to adjust photosystem stoichiometry: effects of the prolonged high-light treatment on pmgA mutants. in Photosynthesis: Mechanisms and Effects (Garab, G. ed.), pp.2929-2932, Kluwer Academic Publishers.

Kamei, A., Ogawa, T. and Ikeuchi, M. (1998) Identification of a novel gene (slr2031) involved in high-light resistance in the cyanobacterium Synechocystis sp. PCC 6803. in Photosynthesis: Mechanisms and Effects (Garab, G. ed.), pp.2901-2905, Kluwer Academic Publishers. [PDF download]

Hihara, Y. and Ikeuchi, M. (1997) Mutation in a novel gene required for photomixotrophic growth leads to enhanced photoautotrophic growth of Synechocystis sp. PCC 6803. Photosynthesis Research, 53: 243-252. [PubMed]

Shen, J.-R., Ikeuchi, M. and Inoue, Y. (1997) Analysis of the psbU gene encoding the 12-kDa extrinsic protein of Photosytem II and studies on its role by deletion mutagenesis in Synechocystis sp. PCC 6803. Journal of Biological Chemistry, 272: 17821-17826. [PubMed] [PDF download]

Umeda, Y., Hirano, A., Hon-Nami, K., Kunito, S., Akiyama, H., Onizuka, T., Ikeuchi, M. and Inoue, Y. (1997) Conversion of CO2 into cellulose by gene manipulation of microalgae: Cloning of cellulose synthase genes from Acetobacter xylinum. in Studies in Surface Science and Catalysis (Delmon, B. and Yates, J.T. eds.), Vol. 114, pp.653-656, Elsevier Science.

Okada, K., Ikeuchi, M., Yamamoto, N., Ono, T-A. and Miyao, M. (1996) Selective and specific cleavage of the D1 and D2 proteins of Photosystem II by exposure to singlet oxygen: factors responsible for the susceptibility to cleavage of the proteins. Biochim. Biophys. Acta 1274: 73-79.

Miyao, M., Yamamoto, N., Ikeuchi, M. and Ono, T. (1995) Specific degradation of the D1 protein of Photosystem II by treatment with hydrogen peroxide in darkness: Implications for the mechanism of degradation of the D1 protein under illumination. Biochemistry 34: 10019-10026.

Ikeuchi, M., Shukla, V.K., Pakrasi, H.B. and Inoue, Y. (1995) Directed inactivation of the psbI gene does not affect Photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Molecular and General Genetics 249: 622-628. [PubMed]

Ikeuchi, M., Inoue, Y. and Vermaas, W.F.J. (1995) Characterization of Photosystem II subunits from the cyanobacterium Synechocystis sp. PCC 6803 in Photosynthesis: from Light to Biosphere (Mathis, P. ed.) Vol. 3, pp.297-300, Kluwer Academic Publishers.

［学会発表］

池内昌彦「ラン藻ゲノムと光合成」2001、日本植物学会（東京）

吉原静恵、小林玲児、耿暁星、池内昌彦「シアノバクテリアSynechocystis sp. PCC 6803の運動の調節に関与する遺伝子クラスターの解析」２００１年９月、日本植物学会（東京）

亀井綾子、湯浅高志、耿暁星、吉原静恵、池内昌彦「Synechocystis sp. PCC 6803におけるSer/Thrプロテインキナーゼ、spkBの解析」２００１年９月、日本植物学会（東京）

岩井雅子、加藤浩、池内昌彦「好熱性シアノバクテリアThermosynechococcus elongatus BP-1の小サブユニットタンパク質PSII-Tの光化学系II二量体化への関与」２００１年９月、日本植物学会（東京）

小林真理、池内昌彦「cpcA変異株の機能を保持した変異フィコシアニンの解析」２００１年９月、日本植物学会（東京）

鈴木布美子、吉原静恵、東正一、渡辺正勝、池内昌彦「Synechocystis sp. PCC 6803の正の走光性の光受容体の遺伝子の解析」２００１年９月、日本植物学会（東京）

相場洋志、池内昌彦、小川晃男、福澤秀哉、園池公毅「シアノバクテリア遺伝子破壊株の網羅的解析―トランスポゾン挿入破壊による全遺伝子産物の機能解析に向けて―」２００１年９月、日本植物学会（東京）

亀井綾子、日原由香子、吉原静恵、耿暁星、金久實、池内昌彦　 Functional Analysis of lexA-like gene, sll1626 in Synechocystis sp. PCC 6803 using DNA microarray. ２００１年８月　第１２回国際光合成会議、ブリスベーン

吉原静恵、小林玲児、耿暁星、池内昌彦　A novel gene involved in phototactic motility of the unicellular cyanobacterium Synechcocystis sp. PCC 6803. ２００１年８月　第１２回国際光合成会議、ブリスベーン

小林真理、加藤浩、池内昌彦　Analysis of mutations to suppress the chloride requirement of the psbV-disruptant of the cyanobacterium Synechocystis sp. PCC 6803. ２００１年８月　第１２回国際光合成会議、ブリスベーン

岩井雅子、加藤浩、池内昌彦　Functional analysis of PSII-T protein of photosystem II complex from the thermophilic cyanobacterium, Thermosynechococcus (formerly Synechococcus) elongatus BP-1. ２００１年８月　第１２回国際光合成会議、ブリスベーン

加藤浩、池内昌彦　Characterization of PSII-I or PSII-K protein-depleted photosystem II from Thermosynechococcus elongatus strain BP-1. PSII-I protein plays an essential role in dimerization of photosystem II. ２００１年８月　第１２回国際光合成会議、ブリスベーン

日原由香子、檜山哲夫、金久實、池内昌彦　DNA microarray analysis of Synechocystis sp. PCC 6803 cells under PSII and PSI light conditions. ２００１年８月　第１２回国際光合成会議、ブリスベーン

相場洋志、亀井綾子、池内昌彦、小川晃男、福澤秀哉、園池公毅　Analysis of a cyanobacterium Synechocystis sp. PCC 6803 genome by application of chlorophyll fluorescence video-imaging system. ２００１年８月　第１２回国際光合成会議、ブリスベーン

福澤秀哉、三浦、松井、入り江、小川晃男、池内昌彦、金子貴一、田畑哲之　Isolation of high-CO2 requiring mutants through the systematic gene disruption system in a cyanobacterium, Synechocystis sp. PCC 6803. ２００１年８月　第１２回国際光合成会議、ブリスベーン

加藤浩、池内昌彦「PSII-I タンパク質は光化学系２複合体の二量体化に必要である」２００１年３月　日本植物生理学会（福岡）

亀井綾子、湯浅高志、耿暁星、折川紅美、吉原静恵、池内昌彦「運動性シアノバクテリアSynechocystis sp. PCC 6803におけるSer/Thr型プロテインキナーゼ(SpkA)の機能解析及び基質タンパク質の同定」２００１年３月　日本植物生理学会（福岡）

亀井綾子、日原由香子、耿暁星、池内昌彦「DNAチップを用いた運動性シアノバクテリアSynechocystis sp. PCC 6803におけるlexA様sll1626遺伝子破壊株の解析」２００１年３月　日本植物生理学会（福岡）

小林真理、加藤浩、池内昌彦「シアノバクテリアSynechocystis sp. PCC 6803のチトクロムC５５０破壊株から得たCl−非要求性変異株の解析」２００１年３月　日本植物生理学会（福岡）

岩井雅子、加藤浩、池内昌彦「好熱性シアノバクテリアSynechococcus elongatusの小サブユニットタンパク質PSII-Tの機能解析」２００１年３月　日本植物生理学会（福岡）

日原由香子、池内昌彦「Synechocystis sp. PCC 6803の異なる光質下での遺伝子発現：DNAマイクロアレイを用いて」２００１年３月　日本植物生理学会（福岡）

相場洋志、池内昌彦、小川晃男、園池公毅「シアノバクテリアSynechocystis PCC6803遺伝子破壊株の蛍光挙動モニタリングによる表現型解析」２００１年３月　日本植物生理学会（福岡）

小林真理、池内昌彦「Synechocystis sp. PCC6803のpsbK破壊株の光／グルコース感受性を抑圧するcpcA変異株の解析」２０００年９月　日本植物学会（静岡）

Katsuhiko OKADA, Masahiko IKEUCHI and Mikio TSUZUKI　Re-examination of the phenotype of psbK mutant in Synechocystis sp. PCC 6803.　Xth International Symposium on Phototrophic Prokaryotes, August 26-31, 2000, Barcelona, Spain.

Hihara, Y., Kamei, A. and Ikeuchi, M.　Analysis of high-light acclimation of a cyanobacterium Synechocystis sp. PCC 6803 using DNA microarray. Xth International Symposium on Phototrophic Prokaryotes, August 26-31, 2000, Barcelona, Spain.

Hiroshi KATOH, Masahiko IKEUCH　Functional analysis of PsbX protein of photosystem II subunit from the thermophilic cyanobacterium Synechococcus elongatus.　Xth International Symposium on Phototrophic Prokaryotes, August 26-31, 2000, Barcelona, Spain.

Ayako KAMEI, Xiao Xing GENG, Kumi ORIKAWA, Masahiko IKEUCHI　Functional analysis of a novel Ser/Thr protein kinase-like gene, spkA, of the motile cyanobacterium Synechocystis sp. PCC 6803.　Xth International Symposium on Phototrophic Prokaryotes, August 26-31, 2000, Barcelona, Spain.

Shizue YOSHIHARA, Xiao Xing GENG, Shinobu OKAMOTO, Kei YURA, Mitiko GO, Takashi MURATA, Masayuki OHMORI, Masahiko IKEUCHI　Mutational analysis of genes involved in motility and transformation of Synechocystis sp. PCC 6803.　Xth International Symposium on Phototrophic Prokaryotes, August 26-31, 2000, Barcelona, Spain.

Masahiko IKEUCHI　Genetic and biochemical analysis of mesophilic and thermophilic cyanobacteria.　２０００年３月　日本植物生理学会（名古屋）

日原由香子, 亀井綾子, 池内昌彦　DNAチップを用いたSynechocystis sp. PCC 6803の強光誘導遺伝子群の解析
２０００年３月　日本植物生理学会（名古屋）

加藤浩、池内昌彦　好熱性シアノバクテリアSynechococcus elongatus の光化学系２サブユニットタンパク質PsbXの機能解析　２０００年３月　日本植物生理学会（名古屋）

亀井綾子、耿暁星、折川紅美、池内昌彦　運動性シアノバクテリアSynechocystis sp. PCC 6803における新規Ser/Thr型プロテインキナーゼ様遺伝子(spkA)の解析　２０００年３月　日本植物生理学会（名古屋）

吉原静恵、耿暁星、岡本忍、由良敬、郷通子、大森正之、池内昌彦　シアノバクテリアSynechocystis sp. PCC 6803における運動と形質転換に関与する遺伝子の機能解析　２０００年３月　日本植物生理学会（名古屋）

岡田克彦、池内昌彦、都筑幹夫　シアノバクテリア Synechocystis sp. PCC6803 の光化学系IIサブユニット psbK 破壊株の強光阻害　２０００年３月　日本植物生理学会（名古屋）

［著書］
大森正之、池内昌彦「シアノバクテリアの光合成遺伝子・蛋白質システムの解明」　タンパク質核酸酵素　2001　pp.2486-2492.
池内昌彦　「植物のゲノム研究プロトコール」　佐々木卓治･田畑哲之･島本功編　2001　pp.197-207.
池内昌彦　「植物生理工学（生物工学基礎コース）」宮地重遠･大森正之編　丸善　1998　pp. 1-22.
池内昌彦　「細胞機能と代謝マップ　?. 細胞の動的機能」日本生化学会編　1998　pp.220-223.
池内昌彦　「細胞機能と代謝マップ　?. 細胞の代謝・物質の動態」日本生化学会編　1997　pp.203-212.
池内昌彦、小林裕和　「植物分子生物学」　山田康之編　朝倉書店　1997 pp.99-151.

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